DNA ligases seal 5′-PO4 and 3′-OH polynucleotide finishes via a few nucleotidyl transfer measures involving ligase-adenylate and DNA-adenylate intermediates. DNA ligases are necessary guardians of genomic integrity, and ligase dysfunction underlies human genetic sickness syndromes. Crystal structures of DNA ligases sure to nucleotide and nucleic acid substrates have illuminated how ligase reaction chemistry is catalyzed, how ligases realize weakened DNA finishes, And the way protein area actions and Lively-site remodeling are utilized to choreograph the top-joining pathway.
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